Harmonized procedures lead to comparable quantification of total oxylipins across laboratories [Research Articles]

Oxylipins are potent lipid mediators involved in a variety of physiological processes. Their profiling has the potential to provide a wealth of information regarding human health and disease and is a promising technology for translation into clinical applications. However, results generated by independent groups are rarely comparable, which increases the need for the implementation of internationally agreed upon protocols. We performed an interlaboratory comparison for the MS-based quantitative analysis of total oxylipins. Five independent laboratories assessed the technical variability and comparability of 133 oxylipins using a harmonized and standardized protocol, common biological materials (i.e., seven quality control plasmas), standard calibration series, and analytical methods. The quantitative analysis was based on a standard calibration series with isotopically labeled internal standards. Using the standardized protocol, the technical variance was within ±15% for 73% of oxylipins; however, most epoxy fatty acids were identified as critical analytes due to high variabilities in concentrations. The comparability of concentrations determined by the laboratories was examined using consensus value estimates and unsupervised/supervised multivariate analysis (i.e., principal component analysis and partial least squares discriminant analysis). Interlaboratory variability was limited and did not interfere with our ability to distinguish the different plasmas. Moreover, ...

Source: The Journal of Lipid Research - November 1, 2020 Category: Lipidology Authors: Mainka, M., Dalle, C., Petera, M., Dalloux-Chioccioli, J., Kampschulte, N., Ostermann, A. I., Rothe, M., Bertrand-Michel, J., Newman, J. W., Gladine, C., Schebb, N. H. Tags: Research Articles Source Type: research