Comparison of acellular small intestinal matrix (ASIM) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide crosslinked ASIM (ASIM-EDC) for repair of full-thickness skin wounds in rabbits

In this study, an acellular small intestinal matrix (ASIM) of bubaline origin was prepared using sodium deoxycholate. In order to reduce the immunogenicity, we crosslinked it using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC). Healing potential of ASIM and ASIM-EDC were compared in full-thickness skin wounds in rabbits. Eighteen clinically healthy New Zealand white rabbits of either sex were randomly divided into three equal groups. Under anesthesia, four full-thickness skin wounds (20×20mm each) were produced on the dorsum of each rabbit. Wounds in control (I) were left open, whereas, in ASIM (II) and ASIM-EDC (III) were repaired with ASIM, and ASIM-EDC, respectively. Planimetry, wound contracture, immunological and histological observations were carried out to evaluate healing process. Significantly (P<0.05) lesser wound contraction was observed in ASIM (II) and ASIM-EDC (III) as compared to control (I). IgG response in sera was significantly (P<0.05) lower over 60 days in ASIM-EDC (III) as compared to ASIM (II) as detected by ELISA. Stimulation index of blood lymphocyte was significantly (P<0.05) lower over 42 days in ASIM-EDC (III) as compared to ASIM (II) by MTT assay. Histologically, improved epithelization, neovascularization, fibroplasia and better arranged collagen fibres were observed in ASIM-EDC (III) as early as on postimplantation day 21. These findings indicate that bubaline ASIM-EDC may have potential for biomedical ap...
Source: Wound Medicine - Category: Journals (General) Source Type: research