A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions

The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.

Source: G3: Genes Genomes Genetics - September 1, 2020 Category: Genetics & Stem Cells Authors: Kim, D.-K., Knapp, J. J., Kuang, D., Chawla, A., Cassonnet, P., Lee, H., Sheykhkarimli, D., Samavarchi-Tehrani, P., Abdouni, H., Rayhan, A., Li, R., Pogoutse, O., Coyaud, E., van der Werf, S., Demeret, C., Gingras, A.-C., Taipale, M., Raught, B., Jacob, Y Tags: Investigations Source Type: research