Evaluation of endogenous control gene(s) for gene expression studies in human blood exposed to 60Co {gamma}-rays ex vivo

In this study, a comparative assessment of the gene expression of six widely used housekeeping endogenous control genes, namely 18S, ACTB, B2M, GAPDH, MT-ATP6 and CDKN1A, was undertaken for a range of 60Co -ray doses (0.5, 1.0, 2.0 and 4.0 Gy) at 8.4 Gy min–1 at 0 and 24 h post-irradiation time intervals. Using the NormFinder algorithm, real-time PCR data obtained from six individuals (three males and three females) were analyzed with respect to the threshold cycle (Ct) value and abundance, Ct pair-wise comparison, intra- and inter-group variability assessments, etc. GAPDH, either alone or in combination with 18S, was found to be the most suitable endogenous control gene and should be used in gene expression studies, especially those involving qPCR of -ray-exposed human blood samples.

Source: Journal of Radiation Research - January 12, 2015 Category: Physics Authors: Vaiphei, S. T., Keppen, J., Nongrum, S., Chaubey, R. C., Kma, L., Sharan, R. N. Tags: Short Communication Source Type: research