Linkage Disequilibrium-Based Inference of Genome Homology and Chromosomal Rearrangements Between Species

The aim of this study was to analyze the genomic homology between cattle (Bos taurus) and buffaloes (Bubalus bubalis) and to propose a rearrangement of the buffalo genome through linkage disequilibrium analyses of buffalo SNP markers referenced in the cattle genome assembly and also compare it to the buffalo genome assembly. A panel of bovine SNPs (single nucleotide polymorphisms) was used for hierarchical, non-hierarchical and admixture cluster analyses. Thus, the linkage disequilibrium information between markers of a specific panel of buffalo was used to infer chromosomal rearrangement. Haplotype diversity and imputation accuracy of the submetacentric chromosomes were also analyzed. The genomic homology between the species enabled us to use the bovine genome assembly to recreate a buffalo genomic reference by rearranging the submetacentric chromosomes. The centromere of the submetacentric chromosomes exhibited high linkage disequilibrium and low haplotype diversity. It allowed hypothesizing about chromosome evolution. It indicated that buffalo submetacentric chromosomes are a centric fusion of ancestral acrocentric chromosomes. The chronology of fusions was also suggested. Moreover, a linear regression between buffalo and cattle rearranged assembly and the imputation accuracy indicated that the rearrangement of the chromosomes was adequate. When using the bovine reference genome assembly, the rearrangement of the buffalo submetacentric chromosomes could be done by SNP BTA ...

Source: G3: Genes Genomes Genetics - July 6, 2020 Category: Genetics & Stem Cells Authors: de Abreu Santos, D. J., Ferreira de Camargo, G. M., Cardoso, D. F., Buzanskas, M. E., Aspilcueta-Borquis, R. R., Hurtado-Lugo, N. A., de Araujo Neto, F. R., Galvao de Albuquerque, L., Ma, L., Tonhati, H. Tags: Investigations Source Type: research