The PAC-3 transcription factor critically regulates phenotype-associated genes in Neurospora crassa

In this study, we used RNA-seq to analyze the phenotypic plasticity induced after pac-3 deletion in the filamentous fungus N. crassa cultivated in media supplemented with sufficient or limited inorganic phosphate. Genes related to morphology, hyphal development, and conidiation were of particular interest in this study. Our results suggest a pac-3 dependency in gene regulation in a Pi-dependent manner. Furthermore, our analysis suggested that the fungus attempts to overcome the deletion effects in a Δ pac-3 mutant through a complex combined regulatory mechanism. Finally, the modulatory responses observed in the Δ pac-3 strain, a double mutant generated based on the Δ mus-52 mutant strain, is strain-specific, highlighting that the phenotypic impact may be attributed to pac-3 absence despite the combined mus-52 deletion.
Source: Genetics and Molecular Biology - Category: Genetics & Stem Cells Source Type: research