Screening Chemical Libraries for Compounds That Affect Protein Sorting to the Yeast Vacuole

Protein trafficking to the yeast vacuole has been extensively studied using a series of deletion mutants. In these genetic screens, mis-targeted vacuolar cargo proteins were used as phenotype markers. Here we describe a similar approach employing pharmacological effects of diverse chemical compounds to mimic molecular phenotypes caused by conventional genetic mutations in protein trafficking genes.
Source: Springer protocols feed by Plant Sciences - Category: Biology Source Type: news