Impairment of bone growth of wistar rat fetuses of diabetic and hypercholesterolemic mothers

Publication date: Available online 31 December 2014 Source:Egyptian Journal of Basic and Applied Sciences Author(s): Hassan I.H. El-Sayyad , Heba A. El-Ghawet , Mohamed S. Al-Haggar , Iman H. Bakr Patients with either diabetes or hypercholesterolemia develop atherosclerosis and impair healing of bone. In the present work we illustrated their role in limb ischemia during bone cells differentiation of Wistar rat fetuses. Pregnant Wistar rats (n = 20 each) were arranged in three groups: control, diabetic or hypercholesterolemic. Diabetes was induced at the fifth day of gestation using streptozotocin, and hypercholesterolemia was carried by feeding virgin rats a diet containing 3% cholesterol for 6 wks prior to the onset of conception. At 13, 15, 17, and 19 d prenatal, pregnant rats were sacrificed, dissected, and fetuses were removed. Hind limbs were separated and subjected to histological and transmission electron microscopic examination, ossification, total calcium content of fetuses, isoenzymes alkaline and acid phosphatase and lactic dehydrogenase electrophoresis and DNA damage. Fetuses of diabetic or hypercholesterolemic mothers exhibited delayed histo-cytological differentiation of chondrocytes, and decreased periosteal ossification. Alkaline and acid phosphatase as well as lactic dehydrogenase isoenzymes showed altered diffusion rate and intensities of their bands reflecting their activities in both diseases comparing with the control. Assessments of bone calc...
Source: Egyptian Journal of Basic and Applied Sciences - Category: Science Source Type: research