The role of EDEM2 versus EDEM1 in ricin transport from the endoplasmic reticulum to the cytosol

In this study we report that EDEM2 is also involved in ricin retrotranslocation out of the ER. However, the role of EDEM1 and EDEM2 in ricin transport to the cytosol seems to differ. EDEM2 promotes ricin retrotranslocation irrespectively of ER translocon accessibility; moreover co- immunoprecipitation and pull-down studies revealed that more ricin can interact with EDEM2 in comparison to EDEM1. On the other hand, interactions of both lectins with RTA are dependent on the structure of ricin A-chain. Thus, our data display a newly discovered role for EDEM2. Moreover, analysis of the involvement of EDEM1 and EDEM2 in ricin retrotranslocation to the cytosol may provide crucial information about general mechanisms of the recognition of ERAD substrates in the ER.

Source: BJ Cell - November 8, 2013 Category: Biochemistry Authors: M Słomińska-Wojewódzka, A Pawlik, I Sokołowska, J Antoniewicz, G Węgrzyn, K Sandvig Tags: BJ Cell Source Type: research