The Response of Human Nasal and Bronchial Organotypic Tissue Cultures to Repeated Whole Cigarette Smoke Exposure

We report on a repeated whole mainstream CS exposure of nasal and bronchial organotypic tissue cultures that mimic the morphological, physiological, and molecular attributes of the human respiratory tract. Despite the similar cellular staining and cytokine secretion in both tissue types, the transcriptomic analyses in the context of biological network models identified similar and diverse biological processes that were impacted by CS-exposed nasal and bronchial cultures. Our results demonstrate that nasal and bronchial tissue cultures are appropriate in vitro models for the assessment of CS-induced adverse effects in the respiratory system and promising alternative to animal experimentation.

Source: International Journal of Toxicology - December 30, 2014 Category: Toxicology Authors: Talikka, M., Kostadinova, R., Xiang, Y., Mathis, C., Sewer, A., Majeed, S., Kuehn, D., Frentzel, S., Merg, C., Geertz, M., Martin, F., Ivanov, N. V., Peitsch, M. C., Hoeng, J. Tags: Regular Articles Source Type: research