The Effect and Mechanism of CXCR4 Silencing on Metastasis Suppression of Human Glioma U87 Cell Line

In this study, we found that CXCR4 silencing could suppress U87 cells invasion and adhesion potential, production of TGF‐β1, IL‐6, and IL‐8, and blocked the G0/G1 phase of the cell cycle. We also found that CXCR4 silencing could up‐regulate the mRNA and protein expression of p53, p21, and E‐cadherin, and down‐regulate the mRNA and protein expression of CD44 and MMP‐2/‐9. Meanwhile, CXCR4 silencing could decrease the phosphorylation of p‐AKT and transcription activity of NF‐κB promoter, and increased the phosphorylation of PTEN. The results provided a new research basis for the further study of CXCR4 gene, the screening of human glioma, as well as the target treatment for glioma and its prognosis. Anat Rec, 2013. © 2013 Wiley Periodicals, Inc.

Source: The Anatomical Record Part B: The New Anatomist - October 28, 2013 Category: Anatomy Authors: Yu Zhu, Ping Yang, Xuebin Zhang, Ling Zhang, Guiping Cui, Qin Wang, Lixia Lv, Yi Zhang, Xin Xin, Tao Yan, Meng Zhao, Nan Zhang Tags: Full Length Article Source Type: research