Fluorescent Pseudomonas strains from mid-mountain water able to release antioxidant proteins directly into water

Publication date: Available online 22 February 2020Source: Microbiological ResearchAuthor(s): Elodie Dussert, Mélissa Tourret, Barbara Deracinois, Matthieu Duban, Valérie Leclère, Benoit Cudennec, Rozenn Ravallec, Josette Behra-MielletAbstractLittle is known about fluorescent Pseudomonas and investigations are needed to help us better understand how their species work. The aim was here to mimic what naturally occurs in environmental water containing strains isolated from mid-mountain water samples and identified as Pseudomonas fluorescens by conventional biochemical techniques. Three strains were cultured before being directly inoculated into distilled water. Surprisingly, the three cell-less extracts obtained after spinning the bacterial suspensions showed strong in vitro anti-oxidative effects against superoxide anion and hydroxyl radical but with discrepancies. The extracts obtained were found to contain antioxidant proteins among other stress proteins that were released by viable bacteria. They were identified using tandem/mass spectrometry and showed different profiles in sodium-dodecyl sulfate polyacrylamide gel electrophoresis. Bacterial identification was deepened using 16S ribonucleic acid and genome sequencing analyses to explain the differences observed between strains.Graphical Abstract
Source: Microbiological Research - Category: Infectious Diseases Source Type: research