From field sampling to pneumatic bioreactor mycelia production of the ectomycorrhizal mushroom Laccaria trichodermophora

Publication date: Available online 21 February 2020Source: Fungal BiologyAuthor(s): Rodolfo E. Ángeles-Argáiz, Ilse A. Carmona-Reyes, Christian Armando Quintero-Corrales, Francisco J. Medina-Macías, Abel Blancas-Cabrera, Norma A. Valdez-Cruz, Miguel Ulloa, Mauricio A. Trujillo-Roldán, Roberto Garibay-OrijelAbstractIn order to increase survival rates of greenhouse seedlings destined for restoration and conservation programs, successful mycorrhization of the seedlings is necessary. To reforest forest ecosystems, host trees must be inoculated with ectomycorrhizal fungi and, in order to guarantee a sufficient supply of ectomycorrhizal inoculum, it is necessary to develop technologies for the mass production of ectomycorrhizal fungi mycelia. We selected the ectomycorrhizal fungus Laccaria trichodermophora, due to its ecological traits and feasible mycelia production in asymbiotic conditions. Here, we report the field sampling of genetic resources, as well as the highly productive nutritional media and cultivation parameters in solid cultures. Furthermore, in order to achieve high mycelial production, we used strain screening and evaluated pH, carbon source concentration, and culture conditions of submerged cultures in normal and baffled shake flasks. The higher productivity culture conditions in shake flasks were selected for evaluation in a pneumatic bioreactor, using modified BAF media with a 10 g/L glucose, pH 5.5, 25º C, and a volumetric oxygen transfer coefficient (KLa) ...

Source: Fungal Biology - February 21, 2020 Category: Biology Source Type: research