Analgesic treatment with buprenorphine should be adapted to the mouse strain

Publication date: Available online 20 February 2020Source: Pharmacology Biochemistry and BehaviorAuthor(s): Juliane Rudeck, Silvia Vogl, Céline Heinl, Matthias Steinfath, Sebastian Fritzw.er, Andrea Kliewer, Stefan Schulz, Gilbert Schönfelder, Bettina BertAbstractBuprenorphine is a commonly used opioid to treat moderate to severe pain in mice. Although strain differences regarding basal pain sensitivity and the analgesic effect of other opioids have been described for mice, the data for buprenorphine is incomplete. Hence, we investigated basal pain sensitivity and the analgesic effect of buprenorphine (0.42, 4.0 mg·kg−1) in male C57BL/6J, Balb/cJ and 129S1/SvImJ mice using the incremental hot plate. Additionally, we verified single nucleotide polymorphisms in Cytochrome P450 3a (Cyp3a) genes, which encode for enzymes that are relevant for buprenorphine metabolism, and analyzed serum and brain concentrations of buprenorphine and its metabolites. Finally, in a pilot survey we determined μ-opioid receptor (MOR) protein expression in whole brain lysates.Basal pain sensitivity differed significantly between the mouse strains (Balb/cJ > C57BL/6J > 129S1/SvImJ). Additionally, buprenorphine showed a dose- and strain-dependent effect: at a higher dose it led to increased antinociception in C57BL/6J and Balb/cJ mice, whereas in 129S1/SvImJ mice this effect was diminished. Serum and brain concentrations of buprenorphine and its metabolites dose-dependently increased and ...
Source: Pharmacology Biochemistry and Behavior - Category: Biochemistry Source Type: research