Expression of lipogenic and milk protein genes in milk fat layer of goat after dietary supplementation of omega-3 rich linseed and chia oils

Publication date: Available online 19 February 2020Source: Small Ruminant ResearchAuthor(s): Ratan K. Choudhary, Jaspal S. Hundal, Manju Wadhwa, Shanti Choudhary, NeetikaAbstractPlant oil supplementation in animal diet is a reliable feeding strategy to enhance omega-3 fatty acids content in milk and meat. To investigate oil induced differential gene expressions of milk protein genes (LALBA, CSN2) and fat synthesis genes (CD36, XDH, ACACA, SCD, FABP3) in mammary epithelial cells, twelve Beetal goats were fed with control diet (CON) and linseed oil (LSO) or chia oil (CO) supplemented diets (1%) for sixty days. Total RNA was isolated from milk fat, a rich source of mammary RNA, before (d 0) and after (d 15 and d 34) of the treatment. Milk RNA quality and quantity did not differ (P > 0.05) either by treatments or by the day of lactation. Real time quantitative PCR (RT-qPCR) analyses showed up-regulation of ACACA, FABP3 and CD36 genes at d 15 in LSO, while expression of SCD and XDH was upregulated at d 34 in CO, with respective to control. Oil supplementation increased abundance of CD36 at d 34 in both the treatment groups (LSO vs. CON; 5-fold, P < 0.01 and CO vs. CON; 4.2-fold, P < 0.05), indicating involvement of CD36 in lipid metabolism. Expression of milk protein genes was though numerically high at d 34 in LSO and CO group (vs. CON), did not differ (P > 0.05) among groups. There were statistically significant interactions observed among all the genes of our st...
Source: Small Ruminant Research - Category: Zoology Source Type: research