Analysis of testis metabolome and transcriptome from the oriental river prawn (Macrobrachium nipponense) in response to different temperatures and illumination times

Publication date: Available online 13 February 2020Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Shubo Jin, Yuning Hu, Hongtuo Fu, Shengming Sun, Sufei Jiang, Yiwei Xiong, Hui Qiao, Wenyi Zhang, Yongsheng Gong, Yan WuAbstractA better understanding of the mechanisms underlying the male sexual differentiation of Macrobrachium nipponense is urgently needed in order to maintain sustainable development of the M. nipponense industry. Environmental factors, especially temperature and illumination, have dramatic effects on gonadal development. The aim of the present study was to identify key genes and metabolites involved in the male sexual differentiation and development of M. nipponense through integrated metabolomics and transcriptome analyses of the testis in response to different temperatures and illumination times. A total of 268 differentially abundant metabolites and 11,832 differentially expressed genes (DEGs) were identified. According to integrated metabolomics and transcriptome analyses, glycerophospholipid and sphingolipid metabolism was predicted to have dramatic effects on the male sexual differentiation and development of M. nipponense. According to the KEGG enrichment analysis of DEGs, oxidative phosphorylation, glycolysis/gluconeogenesis, the HIF-1 signaling pathway, the citrate cycle, steroid hormone synthesis, and the spliceosome complex were predicted to promote male differentiation and development by providing adenosin...

Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - February 13, 2020 Category: Genetics & Stem Cells Source Type: research