Novel ABTS-dot-blot method for the assessment of antioxidant properties of food packaging

Publication date: June 2020Source: Food Packaging and Shelf Life, Volume 24Author(s): Barbara Kusznierewicz, Hanna Staroszczyk, Edyta Malinowska-Pańczyk, Karol Parchem, Agnieszka BartoszekAbstractThe new ABTS-dot-blot method for the direct determination of antioxidant activity of active packaging that is in contact with foodstuffs has been developed. The usefulness of the new method was verified with the use of agarose, pork gelatin, bacterial cellulose and cellulose-chitosan films with incorporated standard antioxidant – Trolox or plant phytochemicals derived from three types of berry juices (chokeberry, blue-berried honeysuckle, rowanberry) or green tea. The plant components used for preparation of films were characterized by antioxidant profiling with the use of HPLC coupled with post-column derivatization with ABTS. The most abundant antioxidants were polyphenols, mainly anthocyanins, hydroxycinnamates and flavan-3-ols. The antioxidant properties of different types of films studied were evaluated by typical cuvette spectrophotometric ABTS test or by the novel method employing ABTS radical stabilised in an agarose gel. The results obtained for the Trolox containing films showed quantitative linear relationship between antioxidant content and the degree of ABTS bleaching in agarose gel. The results of both standard spectrophotometric method as well as ABTS-dot-blot approach indicated that gelatin, bacterial cellulose and chitosan on their own possessed antioxidant activi...
Source: Food Packaging and Shelf Life - Category: Food Science Source Type: research