Metabolomic Investigation of Methicillin-Resistant Staphylococcus aureus

In this study, we describe a workflow focusing on the extraction and quantification of intracellular metabolites of Staphylococcus aureus. A filter-based bacteria sampling system was utilized to separate the media and bacteria; fast quenching with nitrogen was applied to prevent any metabolite leakage; a glass beads beater was used for intracellular metabolite extraction; and the LC-QTOF was combined to quantify the intracellular amino acids of S. aureus. This protocol is demonstrated to be an efficient method for analyzing the intracellular metabolites of S. aureus.
Source: Springer protocols feed by Microbiology - Category: Microbiology Source Type: news