Vitex negundo L. leaf extract inhibits IL-6 and TNF-α secretion and phagocytosis in human leukocytes

Publication date: Available online 5 February 2020Source: Journal of Herbal MedicineAuthor(s): K.W.J.C. Kariyawasam, P.D.N.N. Sirisena, H.L.C. Nanayakkara, W.D. Ratnasooriya, S.M. HandunnettiAbstractVitex negundo L. is used as an anti-inflammatory agent in Ayurveda and Sri Lankan traditional medicine. Here, the anti-inflammatory effects of an aqueous leaf extract (ALE) of V. negundo L. was investigated. The inhibitory effects of freeze-dried ALE (15.7–500 µg/mL) against interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumour necrosis factor-α (TNFα) secretion by human mononuclear cells, phagocytic activity of human neutrophils, and reactive oxygen species (ROS) and nitric oxide (NO) production by human leukocytes were tested in vitro. The inhibitory effects were dose dependent, and the strongest effect was observed at 500 μg/mL (P < 0.05). ALE caused 58.0 ± 1.0% and 26.1 ± 2.0% inhibition of IL-6 and TNFα secretion, respectively, whereas the positive control, Andrographis paniculata, caused complete inhibition. Phagocytic activity and ROS production were inhibited by 55 ± 4.5% and 58.6 ± 8.6%, respectively, whereas the positive controls, Ixora coccinea and diphenyleneiodonium chloride, caused 100 ± 4.4% and 80 ± 6.4% inhibition, respectively. ALE markedly inhibited NO production (87.5 ± 17.7%), and the effect was comparable to that of the positive control, N-monomethyl-L-arginine (95.0 ± 1.0%). The membrane-stabilizing...
Source: Journal of Herbal Medicine - Category: Complementary Medicine Source Type: research