Monitoring Side-Chain Dynamics of Proteins Using 2H Relaxation

Nuclear magnetic resonance (NMR) is a powerful technique capable of monitoring a wide range of motions in proteins on a per residue basis. A variety of 2H relaxation experiments have been developed for monitoring side-chain methyl group motions on the picosecond–nanosecond timescale. These experiments enable determination of the order parameter, S 2 axis, which reports on the rigidity of the C-CH3 bond for side-chain methyl groups. The application of a commonly used subset of these experiments is described in this chapter. It is intended to serve as a practical guide to investigators interested in monitoring side-chain motions.
Source: Springer protocols feed by Protein Science - Category: Biochemistry Source Type: news