Vincristine, carboplatin and cisplatin increase oxidative burst induced by paf in canine neutrophils

Publication date: Available online 24 January 2020Source: Veterinary Immunology and ImmunopathologyAuthor(s): Nathalia Mendez, Pablo Alarcon, Catalina Millán, Rafael A. Burgos, Francisco J. Morera, Javier OjedaAbstractMyelosupression resulting from chemotherapy has been widely described in veterinary medicine; however, there is limited information relating to alterations in neutrophil function after chemotherapy in dogs with cancer. The aim of this study was to determine the non-proliferative effects of vincristine, carboplatin, and cisplatin on canine neutrophils by evaluating activation of oxidative and non-oxidative responses. Neutrophils were isolated from venous blood. Levels of reactive oxygen species (ROS) and metalloproteinase 9 (MMP-9) were measured in vitro during neutrophil exposure to these chemotherapeutic agents for 15 min followed by stimulation with platelet activating factor (PAF). ROS production was detected via luminescence, and MMP- 9 liberation was determined by zymography. The chemotherapeutic agents caused an increase in PAF-induced ROS production, but no change in the non-oxidative response was observed. These results suggest that these chemotherapeutic agents may act as priming agents by increasing the oxidative response. These effects could be beneficial for dogs with cancer by supporting their immune systems; however, excessive ROS liberation has been associated with inflammation, neutrophil-mediated cell injury, carcinogenesis, and metastasis. C...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research