Recombinant β-Glucocerebrosidase specific immunoaffinity ligands selected from phage-displayed combinatorial scFv libraries

Publication date: Available online 22 January 2020Source: Protein Expression and PurificationAuthor(s): R.L. Anisimov, O.A. Ershova, A.V. Ershov, M.A. Filatova, S.A. Katorkin, V.M. SimonovAbstractAntibodies specific to β-Glucocerebrosidase were selected from phage displayed naïve scFv libraries. Biopannings were performed against recombinant human protein β-Glucocerebrosidase immobilized on polystyrene surface, specific phages were eluted with 50% ethylene glycol in citrate buffer (pH 6.0). Several specific binders were discovered and converted to full-size hIgG1 antibodies leading to highly stable binders with dissociation constants (Kd) in the range 10–40 nM. The antibodies were used further as ligands for affinity chromatography, where efficient and selective recovery of biologically active β-Glucocerebrosidase from cultured media of Chinese hamster ovary cells was demonstrated. β-Glucocerebrosidase was purified to nearly homogeneous state and had specific activity comparable to the commercially available preparations (40–44 U/mg protein). The obtained immunoaffinity sorbents have high capacity and can be easily regenerated.

Source: Protein Expression and Purification - January 23, 2020 Category: Biochemistry Source Type: research