Revisiting the Bacterial Mutagenicity Assays: Report by a Workgroup of the International Workshops on Genotoxicity Testing (IWGT)

Publication date: Available online 13 January 2020Source: Mutation Research/Genetic Toxicology and Environmental MutagenesisAuthor(s): Rita Schoeny, Kevin P. Cross, David M. DeMarini, Rosalie Elespuru, Atsushi Hakura, Dan D. Levy, Richard V. Williams, Errol Zeiger, Patricia A. Escobar, Jonathan R. Howe, Masayuki Kato, Jasmin Lott, Martha M. Moore, Stephanie Simon, Leon F. Stankowski, Kei-ichi Sugiyama, Bas-jan M. van der LeedeAbstractThe International Workshop on Genotoxicity Testing (IWGT) meets every four years to obtain consensus on unresolved issues associated with genotoxicity testing. At the 2017 IWGT meeting in Tokyo, four sub-groups addressed issues associated with the Organization for Economic Cooperation and Development (OECD) Test Guideline TG471, which describes the use of bacterial reverse-mutation tests. The strains sub-group analyzed test data from>10,000 chemicals, tested additional chemicals, and concluded that some strains listed in TG471 are unnecessary because they detected fewer mutagens than other strains that the guideline describes as equivalent. Thus, they concluded that a smaller panel of strains would suffice to detect most mutagens. The laboratory proficiency sub-group recommended (a) establishing strain cell banks, (b) developing bacterial growth protocols that optimize assay sensitivity, and (c) testing “proficiency compounds” to gain assay experience and establish historical positive and control databases. The sub-group on criteria for assay...
Source: Mutation Research Genetic Toxicology and Environmental Mutagenesis - Category: Genetics & Stem Cells Source Type: research