Enrichment Reporter System of Genome Editing Positive Cells

This article summarized the design principles and applications of these reporter systems and would provide a reference to construct a more perfecte evaluating system for genome editing.Graphical abstractCo-transfection of genome editing tool plasmid and reporter vector into cells can represent the positive ratio of genome editing cells by expressing functional proteins with the shift of open reading frame induced by the mechanism of genome repair. The following methods with FACS or resistance selection can effectively enrich the positive cells. Eventually, mutation analysis can be done by T7E1 assay or sequencing.
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research