Progress in Monolithic Column-based Separation and Enrichment of Glycoproteins

Publication date: January 2020Source: Chinese Journal of Analytical Chemistry, Volume 48, Issue 1Author(s): Xue WEN, Hai-Jiao ZHENG, Yang ZHANG, Qiong JIAAbstractAs a post-translational modification, protein glycosylation not only reflects on body health, but also serves as the target of drug treatment. Therefore, it is of great significance to study protein glycosylation for the diagnosis and treatment of diseases. However, many glycoproteins of biological importance exhibit relatively low abundance, high complexity, and wide dynamic range in biological samples. It is indispensable to explore highly efficient sample pretreatment methods. In recent years, monolithic columns have attracted extensive attention in the field of separation and enrichment of glycoproteins due to their rapid separation, high efficiency, and good biological compatibility. In this paper, recent progress of monolithic columns in the field of separation and enrichment of glycoproteins is surveyed, and the future development is prospected.Graphical abstractThe separation and enrichment of glycoproteins with low abundance in complex biological systems are critical for the research of glycosylated proteomics. Monolithic column plays an important role in the field of separation and enrichment of glycoproteins due to its advantages of rapid separation, high efficiency, and good biological compatibility. In this paper, recent progress of monolithic columns in the field of separation and enrichment of glycopro...
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research