Isolation and molecular identification of Trichoderma species from wetland soil and their antagonistic activity against phytopathogens

Publication date: Available online 6 January 2020Source: Physiological and Molecular Plant PathologyAuthor(s): Kandasamy Saravanakumar, Myeong-Hyeon WangAbstractTrichoderma species are known to protect the plants from pathogen infections through multifunctions, such as secondary metabolism, mycoparasitism, hyperparasitism, nutrient competition, enzymes and induced systemic resistance (ISR). Herein, we isolated a total of 18 Trichoderma strains including nine species such as T. atroviride, T. virens, T. velutinum, T. harzianum, T. asperellum, T. koningiopsis, T. aureoviride, H. lixii, and T. koningii from the soils samples, collected from the wetland ecosystem of South Korea. These strains were screened against the pathogens- Macrophomina phaseolina (MP), Fusarium graminearum (FG), and Botrytis cinerea (BC) - by in vitro antagonistic assay. Amongst, T. aureoviride (SKCGW013) showed higher antagonistic activity against the targeted pathogens than other isolates did. The strain SKCGW013 was further used for extraction, purification and analysis of the metabolites by using column chromatography (CC) and gas chromatography mass spectroscopy (GC-MS). The expression of secondary metabolites regulatory genes of non-ribosomal peptide synthetase (NRPS), polyketide synthase (PKS) were studied by RT-qPCR. The results showed the presence of eight dominant compounds in the ethyl acetate fraction of the strain SKCGW013 and these compound were then screened by molecular modeling method again...
Source: Physiological and Molecular Plant Pathology - Category: Molecular Biology Source Type: research