Up-regulation of brain cytokines and metalloproteinases 1 and 2 contributes to neurological deficit and brain damage in transient ischemic stroke

Publication date: Available online 28 December 2019Source: Microvascular ResearchAuthor(s): Edna Constanza Gómez Victoria, Eliana Cristina de Brito Toscano, Fabrício Marcus Silva Oliveira, Bárbara Andrade de Carvalho, Marcelo Vidigal Caliari, Antônio Lúcio Teixeira, Aline Silva de Miranda, Milene Alvarenga RachidAbstractIschemic stroke represents a major cause of adult death and severe neurological disability worldwide. Reperfusion following brain ischemia produces an inflammatory cascade that increases brain damage. In this context, matrix metalloproteinases (MMPs) play an important role as pro-inflammatory mediators. The MMP 2 up-regulation seems to promote matrix degradation, blood-brain barrier (BBB) disruption and facilitates the influx of peripheral inflammatory cells to the brain after stroke. However, there are not studies about MMP-1 in this condition. The aim of this study is to evaluate the association of brain damage, inflammatory response and the immunostaining profile of matrix metalloproteinases 1 and 2 after transient global cerebral ischemia. Mice were submitted to bilateral common carotid arterial occlusion (BCCAo) during 25 min. After three days of reperfusion, the neurological deficit score was evaluated and the animals were euthanized. Brain samples were collected in order to analyze the histopathological damage, MMPs 1 and 2 immunostaining and cytokines and chemokines levels. Ischemic group showed neurological deficits associated with brain lesion...
Source: Microvascular Research - Category: Biochemistry Source Type: research