The low levels of nerve growth factor and its upstream regulatory kinases in prion infection is reversed by resveratrol

Publication date: Available online 28 December 2019Source: Neuroscience ResearchAuthor(s): Chao Hu, Cao Chen, Jia Chen, Kang Xiao, Jing Wang, Qi Shi, Yue Ma, LiPing Gao, YueZhang Wu, Lian Liu, Ying Xia, Pu Yan, Adalaiti Maimaitiming, DongHua Zhou, LiNa Zhang, Zhi Bao Chen, Xiao Ping DongAbstractResveratrol shows ability to eliminate prion replication, but the exact mechanism for prion eradication was not clear yet. Our previous studies demonstrate a downregulation of brain-derived nerve growth factor (BDNF) during prion infection, meanwhile recovery of cerebral nerve growth factor (NGF) level by resveratrol treatment has been reported in other neurodegenerative models. To obtain the possible changes of brain NGF and its upstream regulatory cascade during prion infection and after removal of prion propagation, the levels of NGF and its upstream regulatory factors in various prion-infected and prion-eradicated SMB cell lines and mice brains inoculated with various SMB cellular lysates were assessed with various methodologies. The levels of NGF were significantly decreased during prion replication, while recovered after removal of PrPSc by resveratrol in vitro. Morphological assays revealed that the NGF signals mainly colocalized within neurons, but not in the proliferative astrocytes and microglia. The upstream positive regulatory kinases, such as p-CREB, p-CaMKIV, CaMKK2 were decreased in the prion infected cells and mice brains, whereas the negative regulatory one, p-CaMKK2, ...
Source: Neuroscience Research - Category: Neuroscience Source Type: research