Molecular structure study of a heptakis(2,3,6-tri-O–methyl)–β–cyclodextrin complex of cholesterol

Publication date: Available online 19 December 2019Source: SteroidsAuthor(s): Karolína Kučáková, Bohumil DolenskýAbstractHeptakis(2,3,6–tri–O–methyl)–β-cyclodextrin (2) and cholesterol form a water-soluble complex 3. We performed several NMR studies, particularly 1H, 13C, 2D NOESY and DOSY, at various temperatures on 500 and 950 MHz instruments. We discovered that the complex 3 is unstable above 57 °C in heavy water, while it is kinetically stable enough to be studied by NMR in detail at 1 °C. We demonstrated the formation of a face–to–face 2:1 complex with a binding constant of approximately 2.2 × 106 M-2.Graphical abstract
Source: Steroids - Category: Drugs & Pharmacology Source Type: research