Genetic and biochemical variations among sugar beet cultivars resistant to Cercospora leaf spot

Publication date: Available online 17 December 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Bahaa E.S. Abd El-Fatah, Mohamed Hashem, Kamal A.M. Abo-Elyousr, Hadeel M.M. Khalil Bagy, Saad A.M. AlamriAbstractThe goal of the study was to construct a reliable molecular and biochemical tool for the selection of resistant sugar beet cultivars to minimize the loss in productivity caused by Cercospora leaf spot disease. Three PCR marker systems: inter simple sequence repeats (ISSR), and sequence-related amplified polymorphisms (SRAP), and random amplified polymorphic DNA (RAPD) were used to assess the genetic diversity among eight cultivars of sugar beet and to detect the molecular markers linked to resistance to Cercospora beticola. Molecular tools successfully differentiated resistant from susceptible cultivars and detected 10 specific DNA markers in the resistant cultivars. Euclidean distance analysis based on physiological traits grouped the eight cultivars into two main clusters. Protein and isozyme patterns of peroxidase, polyphenol oxidase, chitinase, super oxide dismutase, glutamate oxalo-acetate transaminase and esterase were validated as quick means to select resistant cultivars of sugar beet. Specific protein and isozyme bands were lacking or were induced in cultivars based on their resistance phenotype. Our Results show that molecular markers and isozyme patterns are powerful tools for distinguishing resistant sugar beet cultivars resistant against Ce...
Source: Physiological and Molecular Plant Pathology - Category: Molecular Biology Source Type: research