Synthetically prepared glycooligosaccharides mimicking Candida albicans cell wall glycan antigens – novel tools to study host–pathogen interactions

Abstract
The immunobiological efficacy of synthetically prepared mannooligosaccharides and a glucooligosaccharide mimicking the structure of Candida albicans cell wall glycans was assessed in vivo and in vitro to exploit immune responses. The exposure of mice splenocytes to BSA‐based conjugates of synthetic oligomannosides and oligoglucoside revealed intense influence on T‐cell subset polarization. The conjugates biased the immune responses towards Th1 and Th17 with respect to the prevalence of interferon‐gamma (IFN‐γ) and interleukin (IL)‐17 (IL‐17) over IL‐4 and IL‐10 levels. The inflammatory activity of the conjugates has been evaluated based on the induction of pro‐inflammatory cytokines. Postvaccination, antimannooligosaccharide and antiglucooligosaccharide antisera were subjected to an evaluation of the structure–immunomodulation activity relationship. Clinical isolates of C. albicans CCY 29‐3‐32 and C. albicans CCY 29‐3‐164 were applied to study interactions between Candida cells and anti‐oligosaccharide antibodies. In situ recognition of parietal oligomannosyl and oligoglucosyl sequences in C. albicans cell wall by the antisera raised against BSA‐based conjugates of synthetic oligomannosides and oligoglucoside revealed the effective recognition of specific distribution of natural oligosaccharide sequences in the cell wall of C. albicans serotype A. With respect to these results, it can be concluded that new, synthetically prepared ...

Source: FEMS Yeast Research - August 12, 2013 Category: Research Authors: Ema Paulovičová, Lucia Paulovičová, Ruzena Pilišiová, Slavomir Bystrický, Dmitri V. Yashunsky, Alexander A. Karelin, Yury E. Tsvetkov, Nikolay E. Nifantiev Tags: Research Article Source Type: research