DNA binding reduces the dissociation rate of STAT1 dimers and impairs the interdimeric exchange of protomers

Conclusions:
Our results demonstrate that DNA binding impairs the oscillation rate between STAT1 conformers. Furthermore, these data suggest that the rapid release from high-affinity GAS sites is not a rate-limiting step in IFN?-mediated signal transduction. Further investigations are needed to decipher the physiological significance of the observed dissociation/re-association process of STAT1 dimers.

Source: BMC Biochemistry - December 20, 2014 Category: Biochemistry Authors: Theresa RiebelingJulia StaabChristoph Herrmann-LingenThomas Meyer Source Type: research