IPEC-1 variable immune response to different serovars of Salmonella enterica subsp. enterica

Publication date: Available online 2 December 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Mustapha Berri, Daniel Hogan, Georges Saade, Sylvie Roche, Philippe Velge, Isabelle Virlogeux-Payant, François MeurensAbstractSalmonella is a genus of Gram-negative bacteria in the Enterobacteriaceae family causing various illnesses. The ability of the different serovars of Salmonella enterica subsp. enterica to infect a host and to induce pathology relies in part on their cellular and molecular interactions with the intestinal epithelium. In the current study, an in vitro approach using non-polarized or polarized IPEC-1 porcine intestinal epithelial cells were used in order to assess the relation between adhesion, invasion, and induction of the immune response as a function of the serotype of Salmonella. Five serovars, Choleraesuis (host-adapted), Typhimurium (ubiquitous), Typhisuis (host-restricted), which are relevant for pig infection, and Dublin and Gallinarum, which are host-restricted or host-adapted, were studied. A strong variation was observed in the percentages of adhesion and invasion amongst the S. enterica serovars used to interact with the non-polarized and polarized cells. Subsequently, differences were identified between serovars in terms of immune response induced. Serovars Typhimurium and Typhisuis induced a strong innate immune response four and half hours after the beginning of cell stimulation while Choleraesuis, Gallinarum, and Dublin did not. ...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research