Transcriptomes of the interaction between Fusarium verticillioides and a Streptomyces strain reveal the fungal defense strategy under the pressure of a potential biocontrol agent

Publication date: Available online 27 November 2019Source: Fungal BiologyAuthor(s): C. Strub, C.A.T. Dieye, P.A. Nguyen, F. Constancias, N. Durand, S. Guendouz, M. Pratlong, A. Fontana, S. Schorr-GalindoAbstractThe actinobacteria Streptomyces sp. AV05 isolated from an organic amendment appears to be a potential biocontrol agent (BCA) against mycotoxigenic fungi. It was found to significantly inhibit Fusarium verticillioides growth and mycotoxin production during their co-cultivation on Petri dishes. F. verticillioides growth was durably affected while the decrease of the toxin production levels was reversible, suggesting different BCA actions. The study of both transcriptomes by targeting the second day of confrontation (1 day befor the highest mycotoxin production by the fungus) brought useful information on the interaction involved between F. verticillioides and Streptomyces sp. AV05. RNA-seq data indicated that the dual interaction modified both genetic expression of F. verticillioides and Streptomyces sp. AV05 as 18.5% of the genes were differentially expressed for the fungus against 3.8% for the actinobacteria. Fungal differentially expressed genes (DEGs) were equally up and down regulated while bacterial ones were mainly upregulated. We especially focused the analysis of DEGs on fungal defense reaction to bacterial attack. For example, if this potential BCA implements a strategy of antibiosis with the over expression of 'siderophore-interacting protein' linked to the pr...

Source: Fungal Biology - November 28, 2019 Category: Biology Source Type: research