Development of a biochemical and biophysical suite for integral membrane protein targets: A review

Publication date: Available online 26 November 2019Source: Protein Expression and PurificationAuthor(s): Matthias Haffke, Myriam Duckely, Christian Bergsdorf, Veli-Pekka Jaakola, Binesh ShresthaAbstractThe generation of integral membrane proteins (IMPs) in heterologous systems and their characterization remains a major challenge in biomedical research. Significant efforts have been invested both in academia and in the pharmaceutical industry to establish technologies for the expression, isolation and characterization of IMPs. Here we summarize some of the key aspects, which are important to support structure-based drug design (SBDD) in drug discovery projects. We furthermore include timeline estimates and an overview of the target selection and biophysical screening approaches.

Source: Protein Expression and Purification - November 28, 2019 Category: Biochemistry Source Type: research