Comparative Analysis of Biochemical Biases by Ligation- and Template-Switch-Based Small RNA Library Preparation Protocols [Molecular Diagnostics and Genetics]
CONCLUSIONS:
Our data indicate that miRNAomics from both methods will hardly be comparable. Ligation-based libraries enrich for canonical miRNAs, which thus may be suitable methods for miRNAomics. Template-switch libraries contain increased numbers and different compositions of fragments and long RNAs. Following different interests for other small RNA species, ligation-independent libraries appear to show a more realistic sRNA landscape with lower bias against biochemical modifications.
Source: Clinical Chemistry - Category: Chemistry Authors: Meistertzheim, M., Fehlmann, T., Drews, F., Pirritano, M., Gasparoni, G., Keller, A., Simon, M. Tags: Molecular Diagnostics and Genetics Source Type: research