Abundance of the benthic dinoflagellate Prorocentrum and the diversity, distribution, and diarrhetic shellfish toxin production of Prorocentrum lima complex and P. caipirignum in Japan

Publication date: Available online 25 November 2019Source: Harmful AlgaeAuthor(s): Tomohiro Nishimura, Hajime Uchida, Ryoko Noguchi, Hiroshi Oikawa, Toshiyuki Suzuki, Hiroshi Funaki, Chiho Ihara, Kyoko Hagino, Shingo Arimitsu, Yuta Tanii, Shota Abe, Kana Hashimoto, Katsuya Mimura, Kouki Tanaka, Ippei Yanagida, Masao AdachiAbstractIn the present study, the abundance of Prorocentrum and the molecular phylogeny, distribution, and DST production of P. lima complex and P. caipirignum in Japan were investigated. First, the cell densities of Prorocentrum were assessed from the temperate to subtropical zones in Japan between 2014 and 2018. The cell density in the subtropical zone [19.0 ± 40.2 cells/g wet weight (ww) algae] was significantly higher than that in the temperate zone (1.4 ± 3.4 cells/g ww algae). A total of 244 clonal strains were established from the temperate and subtropical zones. Phylogenetic analyses based on the large-subunit ribosomal DNA D1/D2 revealed that the strains were separated into four species/species complex/phylotypes (P. limacomplex, P. caipirignum, and new phylotypes Prorocentrum spp. types 1 and 2). The strains of P. lima complex could be separated into two clades (1 and 3). Furthermore, the strains of clades 1 and 3 could be separated into nine subclades (1a, 1c, 1d, 1e, 1f, 1 g, 1 h, 1i, and 1 j) and three subclades (3a, 3b, and 3c), respectively. The strains of P. caipirignum were separated into two subclades (b and e). Each phyloty...
Source: Harmful Algae - Category: Environmental Health Source Type: research