Body fluid identification and assignment to donors using a targeted mRNA massively parallel sequencing approach – results of a second EUROFORGEN / EDNAP collaborative exercise

Publication date: Available online 26 November 2019Source: Forensic Science International: GeneticsAuthor(s): S. Ingold, G. Dørum, E. Hanson, D. Ballard, A. Berti, K.B. Gettings, F. Giangasparo, T.M.-L. Kampmann, F.-X. Laurent, N. Morling, W. Parson, C.R. Steffen, A. Ulus, M. van den Berge, K.J. van der Gaag, V. Verdoliva, C. Xavier, J. Ballantyne, C. HaasAbstractIn a previous EUROFORGEN/EDNAP collaborative exercise, we tested two assays for targeted mRNA massively parallel sequencing for the identification of body fluids/tissues, optimized for the Illumina MiSeq/FGx and the Ion Torrent PGM/S5 platforms, respectively. The task of the second EUROFORGEN/EDNAP collaborative exercise was to analyze dried body fluid stains with two different multiplexes, the former Illumina 33plex mRNA panel for body fluid/tissue identification and a 35plex cSNP panel for assignment of body fluids/tissues to donors that was introduced in a proof-of-concept study recently. The coding region SNPs (cSNPs) are located within the body fluid specific mRNA transcripts and represent a direct link between the body fluid and the donor.We predicted the origin of the stains using a partial least squares discriminant analysis (PLS-DA) model, where most of the single source samples were correctly predicted. The mixed body fluid stains showed poorer results, however, at least one component was predicted correctly in most stains. The cSNP data demonstrated that coding region SNPs can give valuable information on...
Source: Forensic Science International: Genetics - Category: Forensic Medicine Source Type: research