Expression and purification of recombinant mouse CRISP4 using a baculovirus system

In this study, we report a three-step purification protocol for expression and purification of mouse CRISP4 protein in High Five™ cells using a baculovirus expression system. Recombinant mouse CRISP4 was recognized by western blotting and structurally characterized using Circular Dichroism (CD). Using the protocol described herein, we generated high yields of soluble and correctly folded recombinant mouse CRISP4.
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research