Resveratrol suppresses nociceptive jaw-opening reflex via 5HT3 receptor-mediated GABAergic inhibition　

Publication date: Available online 9 November 2019Source: Neuroscience ResearchAuthor(s): Kounosuke Hirata, Yuto Nishiki, Ryosuke Goto, Mikito Inagaki, Katsuo Oshima, Yoshihito Shimazu, Mamoru TakedaAbstractSystemic administration of the dietary constituent, resveratrol, was previously shown to inhibit the nociceptive jaw-opening reflex (JOR) via the endogenous opioid system. The present study investigated whether resveratrol could similarly affect the JOR under in vivo conditions via 5 H T3 receptor-mediated GABAergic inhibition. We used electrical stimulation of the tongue in pentobarbital-anesthetized rats to evoke the JOR, which was recorded as the anterior belly of the digastric muscle electromyograms (dEMG). Intravenous administration of resveratrol (2 mg/kg) reduced the dEMG amplitude in response to three times the determined threshold electrical stimulation, with maximum inhibition reached within approximately 10 min. These inhibitory effects on the JOR were reversible to control levels after approximately 20 min. Pretreatment of rats with either 5 H T3 receptor antagonist, ondansetron (0.25-1 mg/kg, i.p.), or GABAA receptor antagonist, bicuculline (0.5-1 mg/kg, i.p.), significantly and dose-dependently attenuated the inhibitory effects of resveratrol on dEMG amplitude compared with untreated controls. These findings suggest that resveratrol also attenuates the nociceptive JOR via 5 H T3 receptor-mediated GABAergic inhibition. The present study t...
Source: Neuroscience Research - Category: Neuroscience Source Type: research