Determination of volatile organic compounds in the stinkhorn fungus Pseudocolus fusiformis in different stages of fruiting body formation

Publication date: Available online 7 November 2019Source: MycoscienceAuthor(s): Pattana Kakumyan, Nakarin Suwannarach, Jaturong Kumla, Natsaran Saichana, Saisamorn Lumyong, Kenji MatsuiAbstractA stinkhorn fungus was collected from the mountainous area of Yoshida campus, Yamaguchi University, Japan. Morphological characterization and similarity of large subunit ribosomal DNA sequences identified the fungus as Pseudocolus fusiformis. MonoTrap™ was combined with gas chromatography-mass spectrometry (GC-MS) to identify volatile organic compounds (VOCs) emitted from the fungus harvested at different stages of maturity. The main VOCs emitted from the mature fruiting body were 3-methyl-butanol, 4-methyl-phenol, and dimethyl tetrasulfide, while none of these compounds were detected in the egg-shaped state. Volatile sulfur-containing compounds, including dimethyl disulfide, trisulfide and tetrasulfide, which are commonly detected in stinkhorn fungi and truffle, were also emitted from this fungus. Furthermore, results elucidated that most VOCs occurred in the mature stage of Ps. fusiformis (fruiting body with arms fuse). This is the first study reporting VOC production of Ps. fusiformis.
Source: Mycoscience - Category: Biology Source Type: research