Experimental detection of proteolytic activity in a signal peptide peptidase of Arabidopsis thaliana

Conclusions:
The data from the in vitro cell-free assay indicated that the membrane fraction of both Arabidopsis cells and AtSPP recombinantly expressed in yeast actually possessed proteolytic activity for a human SPP substrate. We concluded that plant SPP possesses proteolytic activity and may be involved in RIP.

Source: BMC Biochemistry - July 6, 2013 Category: Biochemistry Authors: Masako HoshiYu OhkiKeisuke ItoTaisuke TomitaTakeshi IwatsuboYoshiro IshimaruKeiko AbeTomiko Asakura Source Type: research