A novel interplay between the ubiquitin-proteasome system and serine proteases during Drosophila development

The concentrations of the Drosophila proteasomal and extraproteasomal polyubiquitin receptors fluctuate in a developmentally regulated fashion. This fluctuation is generated by a previously unidentified proteolytic activity. Here we describe the purification, identification and characterization of this protease (endoproteinase I). Its expression increases sharply at L1-L2 larval stages, remains high until the second half of L3 stage then declines dramatically. This sharp decrease coincides precisely with the increase of polyubiquitin receptor concentrations in late L3 larvae, which suggests a tight developmental co-regulation. RNAi-induced downregulation of endoproteinase I results in pupal lethality. Interestingly, we found a cross-talk between the 26S proteasome and this larval protease: transgenic overexpression of the in vivo target of endoproteinase I, the C-terminal half of the proteasomal polyubiquitin receptor subunit p54/Rpn10 results in transcriptional downregulation of endoproteinase I, and consequently a lower level of proteolytic elimination of the polyubiquitin receptors. Another larval protease, Jonah65A-IV, which degrades only unfolded proteins and exhibits similar cross-talk with the proteasome has also been purified and characterized. It may prevent the accumulation of polyubiquitylated proteins in larvae contrary to the low polyubiquitin receptor concentration.

Source: BJ Cell - June 28, 2013 Category: Biochemistry Authors: Z Lipinszki, E Klement, E Hunyadi-Gulyas, K F Medzihradszky, R Márkus, M Pál, P Deák, A Udvardy Tags: BJ Biomolecules Source Type: research