Endoplasmic reticulum stress induced apoptosis and caspase activation is mediated through mitochondria during megakaryocyte differentiation

Publication date: Available online 25 October 2019Source: MitochondrionAuthor(s): Narasaiah Kovuru, Sanjeev Raghuwanshi, Durga Shankar Sharma, Swati Dahariya, Adithya Pallepati, Ravi Kumar GuttiAbstractMegakaryocytopoiesis involves the process of the development of hematopoietic stem cells into megakaryocytes (MKs), which are the specialized cells responsible for the production of blood platelets. Platelets are one of the crucial factors for hemostasis and thrombosis. In terminally differentiated MKs many molecular process such as caspase activation and a massive cytoskeletal rearrangement drive the formation of cytoplasmic extensions called proplatelets. These cytoplasmic extensions packed with granules and organelles are then released from the bone marrow into the blood circulation as platelets. Classically, caspase activation is associated with apoptosis and recent reports suggest their involvement in cell differentiation and maturation. There is no clear evidence demonstrating the stimulus for caspase activation during megakaryocyte development. In the current study, we attempted to understand the importance of endoplasmic reticulum stress in the caspase activation during megakaryocyte maturation. We used human megakaryoblstic cell line (Dami cells) as an experimental model. We induced these cells with PMA (Phorbol 12-myristate 13-acetate) a known inducer of megakaryocyte to understand the involvement of ER stress during maturation, with increased cell size in association...

Source: Mitochondrion - October 25, 2019 Category: Biochemistry Source Type: research