Disturbance of mitochondrial functions associated with permeability transition pore opening induced by cis-5-tetradecenoic and myristic acids in liver of adolescent rats

Publication date: Available online 23 October 2019Source: MitochondrionAuthor(s): Cristiane Cecatto, Alexandre Umpierrez Amaral, Alessandro Wajner, Simone Magagnin Wajner, Roger Frigério Castilho, Moacir WajnerAbstractPatients affected by very long-chain acyl-CoA dehydrogenase (VLCAD) deficiency commonly present liver dysfunction whose pathogenesis is poorly known. We demonstrate here that major metabolites accumulating in this disorder, namely cis-5-tetradecenoic acid (Cis-5) and myristic acid (Myr), markedly impair mitochondrial respiration, decreasing ATP production in liver mitochondrial preparations from adolescent rats. Other parameters of mitochondrial homeostasis such as membrane potential (ΔΨm) and Ca2+ retention capacity were strongly compromised by these fatty acids, involving induction of mitochondrial permeability transition. The present data indicate that disruption of mitochondrial bioenergetics and Ca2+ homeostasis may contribute to the liver dysfunction of VLCAD deficient patients.
Source: Mitochondrion - Category: Biochemistry Source Type: research