Increased acute immune response during the meningo-encephalitic stage of Trypanosoma brucei rhodesiense sleeping sickness compared to Trypanosoma brucei gambiense

Publication date: Available online 15 December 2014 Source:Translational Proteomics Author(s): Natalia Tiberti , Veerle Lejon , Dieudonné Mumba Ngoyi , Enock Matovu , John Enyaru , Nadia Walter , Catherine Fouda , Pascal Lutumba , Krister Kristensson , Sylvie Bisser , Joseph Mathu Ndung’u , Philippe Büscher , Jean-Charles Sanchez The host central nervous system (CNS) response to infection with Trypanosoma brucei (T. b.) gambiense or T. b. rhodesiense parasites, the causing agent of human African trypanosomiasis (HAT), is a poorly explored area. The two parasites are responsible for respectively a chronic and an acute form of HAT. In both cases, however, the disease progresses from a haemolymphatic first stage (S1) to a meningo-encephalitic second stage (S2) due to the penetration of parasites into the CNS. In the present study, we investigated and compared the cerebrospinal fluid (CSF) from S2 patients affected by either T. b. gambiense or T. b. rhodesiense HAT, using a mass spectrometry quantitative proteomics approach. Gene ontology and pathway analyses on the 222 quantified human proteins, revealed a predominant activation of the innate immune and the acute phase responses in rhodesiense HAT patients. These results were further confirmed through the verification of the over-expression of two proteins involved in these mechanisms, C-reactive protein (CRP) and orosomucoid 1 (ORM1), in 126 S2 HAT patients suffering from either the chronic or the acute for...
Source: Translational Proteomics - Category: Biomedical Science Source Type: research