In Vivo Electrochemical Detection of Hydrogen Peroxide and Dopamine

Publication date: October 2019Source: Chinese Journal of Analytical Chemistry, Volume 47, Issue 10Author(s): Shuai ZHANG, Tao-Tao FENG, Li ZHANG, Mei-Ning ZHANGAbstractSimultaneous analysis of hydrogen peroxide (H2O2) and dopamine (DA) in central nervous system is of great significance and has great challenges. Herein, we reported one ring-disk microelectrode for simultaneous electrochemical analysis of H2O2 and DA. To selectively detect H2O2, the carbon fiber disk (CFdisk) electrode in the middle of the ring-disk microelectrode was electrochemically modified by Prussian blue (PB) and poly(2,3-dihydrothieno-1,4-dioxin) (PEDOT). The Auring on the periphery of the ring-disk microelectrode was used to detect DA. The ring-disk microelectrode exhibited sensitive response to H2O2 (linearity range: 1–29 μM) and DA (linearity range: 0.5–25 μM) with a detection limit of 0.4 μM and 0.18 μM, respectively. The ring-disk microelectrodes were successfully used in measuring H2O2 and DA in vivo. This method provided a new platform for the study of neurophysiology and pathology of H2O2 and DA.Graphical AbstractOne ring-disk microelectrode for simultaneous electrochemical analysis of hydrogen peroxide (H2O2) and dopamine (DA) was reported. The ring-disk microelectrode exhibited sensitive response to H2O2 and DA without interference with each other. The microelectrodes were successfully used in measuring H2O2 and DA in vivo, which provides a new platform for the study of neurophysiology...

Source: Chinese Journal of Analytical Chemistry - October 10, 2019 Category: Chemistry Source Type: research