Polymerase chain reaction optimization for amplification of Guanine-Cytosine rich templates using buccal cell DNA

Conclusions: It was possible to amplify the GC rich region of FMR 1 gene with reproducibility in the presence of betaine and DMSO as additives without the use of commercially available kits for DNA extraction and the expensive thermostable polymerases.

Source: Indian Journal of Human Genetics - June 4, 2013 Category: Genetics & Stem Cells Authors: C. H. W. M. R. Chandrasekara BhagyaWS Wijesundera SulochanaN Perera Hemamali Source Type: research