Phenotypic characterization of immune cells in fetal tissues of cattle immunized and challenged with Neospora caninum

Publication date: Available online 1 October 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): J.E. Maldonado Rivera, J. DeLay, Y.P. Hecker, M.G. Monterubbianesi, G.J. Cantón, C.M. Campero, A.C. Odeón, D.P. MooreAbstractThe purpose of this work was to characterize the cellular phenotype in inflammatory infiltrates of fetal tissues from pregnant heifers immunized and experimentally challenged with Neospora caninum. Fetuses from 20 heifers separated into 5 groups were obtained. The experiment was designed as follow: Group A, heifers inoculated intravenously with live tachyzoites of Argentine strain NC-6 (n = 4); Group B heifers inoculated subcutaneously with soluble native antigen from the same strain formulated with immune stimulant complexes (ISCOMs) (n = 4); Group C heifers inoculated with recombinant proteins, rNcSAG1, rNcHSP20, rNcGRA7 formulated with ISCOMs (n = 4), Group D heifers inoculated subcutaneously with sterile phosphate buffered solution (n = 4) and Group E heifers inoculated subcutaneously with antigen-free ISCOMs (n = 4). Experimental challenge was performed at 70 days of gestation and all heifers were euthanized 34 days later. Fetal tissues were taken for histological studies. Inflammatory lesions were observed in brain and lung, and immunhistochemistry was used to identify CD3+, CD20+ and MHC II+ cells. The majority of the cells that infiltrate and circumscribe the lesions in the brain and lung tissue expressed MHC II anti...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research