Amplicon Sequencing-Based Noninvasive Fetal Genotyping for RHD-Positive D Antigen-Negative Alleles [Molecular Diagnostics and Genetics]

CONCLUSIONS: This method is a reliable noninvasive fetal RHD genotyping method for Japanese and other East Asian populations. The genotyping principle of amplifying 2 different regions using the same primer pair and distinguishing them by their sequence difference during the subsequent mapping procedure is also theoretically applicable to RHD-positive D antigen-negative alleles prevalent in Africans. Therefore, this method offers an opportunity to consider targeted administration of anti-D immunoglobulin to RhD-negative pregnant women in East Asian and African countries and to increase the specificity of the fetal RHD genotyping implemented nationwide in several European countries.

http://clinchem.aaccjnls.org/cgi/content/short/65/10/1307?rss=1

Source: Clinical Chemistry - September 29, 2019 Category: Chemistry Authors: Takahashi, K., Migita, O., Sasaki, A., Nasu, M., Kawashima, A., Sekizawa, A., Sato, T., Ito, Y., Sago, H., Okamoto, A., Nakabayashi, K., Hata, K. Tags: Molecular Diagnostics and Genetics Source Type: research